HIV-1 Fusion Assay
نویسندگان
چکیده
منابع مشابه
Fluorescence resonance energy transfer-based HIV-1 virion fusion assay.
The fluorescence resonance energy transfer (FRET)-based HIV-1 virion fusion assay exploits the incorporation of beta-lactamase-Vpr chimeric proteins into HIV-1 virions and their subsequent delivery into the cytoplasm of target cells as a marker of fusion. This transfer can be monitored by the enzymatic cleavage of the CCF2-AM dye, a fluorescent substrate of beta-lactamase (BlaM), loaded into th...
متن کاملHIV-1 Membrane Fusion
The AIDS pandemic is spreading unchecked in many parts of the world. Currently available anti-viral regimens are plagued by their high cost, by serious side effects in a significant minority of recipients, and by the increasing problems of drug-resistant HIV variants. Fortunately, rapid advances in understanding how HIV-1 enters cells have lead to the identification of promising new drug target...
متن کاملDevelopment of a rapid cell-fusion-based phenotypic HIV-1 tropism assay
INTRODUCTION A dual split reporter protein system (DSP), recombining Renilla luciferase (RL) and green fluorescent protein (GFP) split into two different constructs (DSP1-7 and DSP8-11), was adapted to create a novel rapid phenotypic tropism assay (PTA) for HIV-1 infection (DSP-Pheno). METHODS DSP1-7 was stably expressed in the glioma-derived NP-2 cell lines, which expressed CD4/CXCR4 (N4X4) ...
متن کاملAlpha-complementation assay for HIV envelope glycoprotein-mediated fusion.
The fusion reaction mediated by viral envelope glycoproteins proceeds through an ordered series of conformational changes in the envelope glycoprotein. Fusion inhibitors have been developed that target glycoprotein subunits, arresting the reaction at different points in the process. We report the development of a novel method for detecting viral glycoprotein-mediated fusion that is based on the...
متن کاملdesign of small molecules with hiv fusion inhibitory property based on gp41 interaction assay
background: gp41 of hiv (human immunodeficiency virus) is a protein that mediates fusion between viral and cellular membranes. the agent, t-20, which has been approved for hiv inhibition, can restrain gp41 function in the fusion process; nevertheless, it has disadvantages like instability, high cost of production and injection form to be delivered twice a day. methods: several molecules like nb...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2014
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1212